Cytokeratin 19 antibody (AA 352-368)

Catalog No. ABIN215749

Product Details anti-Cytokeratin 19 Antibody

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Binding Specificity: AA 352-368

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cytokeratin 19 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: Antigen Recognized in Human, rat, fish, bovine, Species (tested so far) not with mouse, woodchuck, chicken, xenopus. Reactivities on Cultured Cell Lines: MCF-7, RT 112, Detroit 562, RPMI 2650, HT-29, SSC-12. Polypeptide Reacting: Mr 40 000 polypeptide (cytokeratin 19) of human glandular epithelia. The epitope has been localized on aa352-368 (VRADSERQNQEYQRLMD) of the a-helical fragment.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%) Marmoset, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (88%) Mouse, Horse (82%).

Purification: Protein A purified

Immunogen: Cytokeratin 19 of Mr 40000, from cultured human MCF-7 cells. Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey (100%), Marmoset, Rat, Hamster, Panda, Dog, Bovine, Rabbit, Pig (88%), Mouse, Horse (82%).

Isotype: IgG2b

Application Details

Application Notes: Approved: ELISA, IHC, IHC-Fr, WB

Usage: Suitable for Immunohistochemistry (frozen tissue), Western Blot (Western) and ELISA. Immunohistochemistry: 1:10 with PBS,  pH 7.4, Incubation Time 1 hr at RT.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: deionized water

Concentration: Lot specific

Buffer: Lyophilized.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: Add glycerol (40-50%) -20°C.

Target Details for Cytokeratin 19

Target: Cytokeratin 19 (KRT19) (Keratin 19 (KRT19))

Alternative Name: KRT19 / CK19 / Cytokeratin 19 (KRT19 Products)

Synonyms: CK19 antibody, K19 antibody, K1CS antibody, AI663979 antibody, EndoC antibody, Krt-1.19 antibody, Krt1-19 antibody, Ka19 antibody, k19 antibody, ck19 antibody, k1cs antibody, krt9 antibody, krt15 antibody, MGC76282 antibody, GK-19 antibody, MGC83069 antibody, KRT19 antibody, keratin 19 antibody, keratin 19 L homeolog antibody, keratin, type I cytoskeletal 19 antibody, KRT19 antibody, Krt19 antibody, krt19 antibody, krt19.L antibody, LOC100344434 antibody, LOC101117946 antibody

Background: Name/Gene ID: KRT19

Synonyms: KRT19, CK19, Cytokeratin 19, Cytokeratin-19, Keratin 19, Keratin-19, CK-19, K19, K1CS, Keratin, type I, 40-kd

Gene ID: 3880

UniProt: P08727