Estrogen Receptor alpha antibody (AA 1-21)

Catalog No. ABIN215829

The Rabbit Polyclonal anti-Estrogen Receptor alpha antibody (ABIN215829) specifically detects Estrogen Receptor alpha in WB, IHC, IHC (p), ICC and IHC (fro). The antibody is reactive with Human, Mouse, Rat, Rabbit, Monkey, Horse and Hamster samples.

Quick Overview for Estrogen Receptor alpha antibody (AA 1-21) (ABIN215829)

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Reactivity: Human, Mouse, Rat, Rabbit, Monkey, Horse, Hamster

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Estrogen Receptor alpha antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Estrogen Receptor alpha Antibody

Binding Specificity: AA 1-21

Specificity: Recognizes human and rat endogenous ER alpha. Detects a band of ~64kD by Western blot. Does not cross-react with ER beta. This sequence is completely conserved in rat and mouse ER alpha and is highly conserved in sheep, porcine, canine, bovine and chicken ER alpha.

Predicted Reactivity: Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Tamarin, Mouse, Rat, Hamster, Cat, Rabbit, Horse (100%) Panda, Bovine, Pig (95%) Sheep, Opossum (90%) Turkey, Chicken (86%).

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide corresponding to aa 1-21 (M1TMTLHTKASGMALLHQIQGN21) of N-terminal human ER (estrogen receptor ). This sequence is completely conserved in rat and mouse ER and is highly conserved in sheep, pig, dog, bovine and chicken ER. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Tamarin, Mouse, Rat, Hamster, Cat, Rabbit, Horse (100%), Panda, Bovine, Pig (95%), Sheep, Opossum (90%), Turkey, Chicken (86%).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ICC, IHC, IHC-Fr, IHC-P, WB (1 μg/mL)

Usage: Suitable for use in Immunocytochemistry, Immunohistochemistry and Western Blot. Immunohistochemistry (frozen, paraffin sections):1-5 μg/mL. Western Blot:1 μg/mL.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Epitope-affinity purified IgG in PBS, 1 mg/mL BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C
Long term: -20°C.

Target Details for Estrogen Receptor alpha

Target: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Alternative Name: ER Alpha / Estrogen Receptor

Background: Name/Gene ID: ESR1
Subfamily: NR3 Steroid receptor
Family: NHR

Synonyms: ESR1, Era, ERalpha, Estrogen receptor, Estradiol receptor, ER-alpha, Estrogen receptor 1, NR3A1, ER, ESR, ESRA, Estrogen receptor alpha

Gene ID: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly