Rubella Virus Glycoprotein E1 (AA 801-900) antibody (Alexa Fluor 555)
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- Target
- Rubella Virus Glycoprotein E1
- Binding Specificity
- AA 801-900
- Reactivity
- Rubella Virus (RuV), Virus
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Alexa Fluor 555
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Application
- Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))
- Cross-Reactivity
- Virus
- Cross-Reactivity (Details)
- Rubella virus
- Purification
- Purified by Protein A.
- Immunogen
- KLH conjugated synthetic peptide derived from Rubella Virus E1 envelope glycoprotein
- Isotype
- IgG
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- Application Notes
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IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200 - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 1 μg/μL
- Buffer
- Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.
- Preservative
- ProClin
- Precaution of Use
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
- Storage
- -20 °C
- Storage Comment
- Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.
- Expiry Date
- 12 months
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- Target
- Rubella Virus Glycoprotein E1
- Alternative Name
- E1 glycoprotein
- Target Type
- Viral Protein
- Background
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Synonyms: E1, E1 envelope glycoprotein, Glycoprotein E1, Spike glycoprotein E1, POLS_RUBVM.
Background: Rubella virus (RV), the sole member of the genus Rubivirus within the family Togaviridae, is a small enveloped, positive strand RNA virus. The nucleocapsid consists of 40S genomic RNA and a single species of capsid protein which is enveloped within a host-derived lipid bilayer containing two viral glycoproteins, E1 (58 kDa) and E2 (42-46 kDa). In virus infected cells, RV matures by budding either at the plasma membrane, or at the internal membranes depending on the cell type and enters adjacent uninfected cells by a membrane fusion process in the endosome, directed by E1-E2 heterodimers. The heterodimer formation is crucial for E1 transport out of the endoplasmic reticulum to the Golgi and plasma membrane. In RV E1, a cysteine at position 82 is crucial for the E1-E2 heterodimer formation and cell surface expression of the two proteins. E1 has been shown to be a type 1 membrane protein, rich in cysteine residues with extensive intramolecular disulphide bonds [PMID: 11682134]
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