HPV16 E7 antibody (Middle Region) (Alexa Fluor 647)

Catalog No. ABIN2173799

Product Details anti-HPV16 E7 Antibody (Alexa Fluor 647)

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Binding Specificity: Middle Region

Reactivity: Human Papillomavirus 16 (HPV-16)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HPV16 E7 antibody is conjugated to Alexa Fluor 647

Application: Western Blotting (WB), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc))

Specificity: This antibody may also detect HPV35-E7

Cross-Reactivity: Human, Virus

Cross-Reactivity (Details): HPV16

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from middle of HPV16-E7

Isotype: IgG

Application Details

Application Notes: IF(IHC-P) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for HPV16 E7

Target: HPV16 E7 (HPV-16 E7) (Human Papilloma Virus 16 E7 (HPV-16 E7))

Abstract: HPV-16 E7 Products

Synonyms: transforming protein E7 antibody, E7 antibody

Target Type: Viral Protein

Background:

Synonyms: Protein E7, E7

Background: Plays a role in viral genome replication by driving entry of quiescent cells into the cell cycle. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. E7 protein has both transforming and trans-activating activities. Induces the disassembly of the E2F1 transcription factor from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Interferes with host histone deacetylation mediated by HDAC1 and HDAC2, leading to transcription activation. Plays also a role in the inhibition of both antiviral and antiproliferative functions of host inteferon alpha.

Gene ID: 1489079

UniProt: P03129