ERI1 antibody (AA 51-150) (HRP)

Catalog No. ABIN2175651

Product Details anti-ERI1 Antibody (HRP)

Target: ERI1 (Exoribonuclease 1 (ERI1))

Binding Specificity: AA 51-150

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERI1 antibody is conjugated to HRP

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Cross-Reactivity: Mouse

Predicted Reactivity: Human,Rat,Dog,Cow,Sheep,Pig,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human HEXO

Isotype: IgG

Application Details

Application Notes: IHC-P 1:200-400
IHC-F 1:100-500

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Do NOT add Sodium Azide! Use of Sodium Azide will inhibit enzyme activity of horseradish peroxidase.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for ERI1

Target: ERI1 (Exoribonuclease 1 (ERI1))

Alternative Name: HEXO (ERI1 Products)

Synonyms: THEX1 antibody, thex1 antibody, zgc:110635 antibody, hexo antibody, Eri-1 antibody, 3'HEXO antibody, HEXO antibody, 3'hexo antibody, 3110010F15Rik antibody, Thex1 antibody, eri-1 antibody, RGD1308378 antibody, exoribonuclease 1 antibody, three prime repair exonuclease 1 antibody, exoribonuclease 1 L homeolog antibody, 3'-5' exonuclease eri-1 antibody, ERI1 antibody, CpipJ_CPIJ003945 antibody, eri1 antibody, eri1.L antibody, Eri1 antibody, eri-1 antibody

Background:

Synonyms: HEXO, THEX1, 3'HEXO, 3'-5' exoribonuclease 1, 3'-5' exonuclease ERI1, Eri-1 homolog, Histone mRNA 3'-end-specific exoribonuclease, Histone mRNA 3'-exonuclease 1, Protein 3'hExo, ERI1, 3'EXO

Background: RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Gene ID: 90459

UniProt: Q8IV48