TNF alpha antibody

Catalog No. ABIN2191873

This Mouse Monoclonal antibody specifically detects TNF alpha in WB, FACS, IHC (p) and IHC (fro). It exhibits reactivity toward Human. It has been mentioned in 4+ publications

Quick Overview for TNF alpha antibody (ABIN2191873)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 52B83

Product Details anti-TNF alpha Antibody

Cross-Reactivity (Details): Cross reactivity: Mouse : Yes, Guinea pig : Yes, Rhesus monkey : Yes

Sterility: 0.2 μm filtered

Isotype: IgG1

Application Details

Application Notes: For immunohistochemistry, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. 1

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.

References for anti-TNF alpha antibody (ABIN2191873)

van der Laan, de Leij, Buurman, Timens, ten Duis: "Tumor necrosis factor alpha (TNFalpha) in human skin: a comparison of different antibodies for immunohistochemistry." in: Archives of dermatological research, Vol. 293, Issue 5, pp. 226-32, (2001) (PubMed).

Gerspach, Götz, Zimmermann, Kolle, Böttinger, Grell: "Detection of membrane-bound tumor necrosis factor (TNF): an analysis of TNF-specific reagents." in: Microscopy research and technique, Vol. 50, Issue 3, pp. 243-50, (2000) (PubMed).

Bradding, Mediwake, Feather, Madden, Church, Holgate, Howarth: "TNF alpha is localized to nasal mucosal mast cells and is released in acute allergic rhinitis." in: Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, Vol. 25, Issue 5, pp. 406-15, (1995) (PubMed).

Bradding, Roberts, Britten, Montefort, Djukanovic, Mueller, Heusser, Howarth, Holgate: "Interleukin-4, -5, and -6 and tumor necrosis factor-alpha in normal and asthmatic airways: evidence for the human mast cell as a source of these cytokines." in: American journal of respiratory cell and molecular biology, Vol. 10, Issue 5, pp. 471-80, (1994) (PubMed).

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor-alpha

Background: The monoclonal antibody 52B83 reacts with tumor necrosis factor alpha (TNF-alpha). TNF-alpha is a homotrimeric 17 kDa protein, that interacts with either one of the two types of TNF-receptors, termed I and II, leading to receptor cross-linking and signal transduction. The receptors differ strongly in their intra-cellular signaling pathways. TNF-alpha was originally described as a highly cytotoxic cytokine for tumor cells, it causes tumor necrosis in vivo and shows cytolytic activity against tumor cells in vitro. Furthermore, TNF-alpha is found to be a central mediator in many inflammatory and immunological processes. It can be induced by various products of micro-organisms and by various cytokines leading to expression of a wide variety of cytokines. The pro-inflammatory properties of TNF-alpha play a central role in several auto-immune diseases such as rheumatoid arthritis and inhibition by neutralizing molecules have been shown to be beneficial in patients. Aliases TNF, TNF-SF2, cachectin

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome