Beta3 Integrin Subunit antibody
Reactivity: Human
IP, Func, IHC (fro), IA
Host: Mouse
Monoclonal
BV4
unconjugated
(2 references)-
- Target
- Beta3 Integrin Subunit
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- Un-conjugated
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Application
- Immunoprecipitation (IP), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunoassay (IA)
- Cross-Reactivity (Details)
- Cross reactivity: Bovine : Yes
- Sterility
- 0.2 μm filtered
- Clone
- BV4
- Isotype
- IgG1
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- Application Notes
- For immunohistochemistry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user's experimental setting. Positive Endometrium control
- Restrictions
- For Research Use only
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- Buffer
- PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C
- Storage Comment
- Product should be stored at 4 °C. Under recommended storage conditions, product is stable for at least one year. The exact expiry date is indicated on the label.
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: "Alpha-v-beta3 integrin expression in melanocytic nevi and cutaneous melanoma." in: Journal of cutaneous pathology, Vol. 34, Issue 11, pp. 851-6, (2007) (PubMed).
: "Role of alphavbeta3 integrin in the activation of vascular endothelial growth factor receptor-2." in: The EMBO journal, Vol. 18, Issue 4, pp. 882-92, (1999) (PubMed).
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: "Alpha-v-beta3 integrin expression in melanocytic nevi and cutaneous melanoma." in: Journal of cutaneous pathology, Vol. 34, Issue 11, pp. 851-6, (2007) (PubMed).
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- Target
- Beta3 Integrin Subunit
- Background
- The monoclonal antibody BV4 recognizes human beta3 integrin subunit present in Platelet glycoprotein GPIIb-IIIa (integrin alphaIIb/beta3, CD41/CD61) and in the vitronectin receptor (integrin alphaV/beta3, CD51/CD61). Intergins are a family of heterodimeric membrane glycoproteins expressed on diverse cell types which function as the major receptors for extracellular matrix and as cell-cell adhesion molecules. As adhesion molecules they play an important role in numerous biological processes such as platelet aggregation, inflammation, immune function, wound healing, tumour metastasis and tissue migration during embryogenesis. In addition integrins are involved in signaling pathways, transmitting signals both into an out from cells. All integrins consist of two non- covalently associated subunits, alpha and beta. At least 12 different alpha subunits and 8 beta subunits have been identified. The beta subunits all contain 56 conserved cysteines (except beta4 which has 48) which are arranged in four repeating units. The beta3 subunit is a 93 kDa protein that contains a large loop in the N-terminus stabilized by intrachain disulphide bonding with the first cysteine-rich repeat. Platelet glycoprotein GPIIb-IIIa is expressed on platelets and megakaryoblasts. It is constitutively expressed and becomes activated on triggered platelets. Platelet glycoprotein GPIIb-IIIa binds to fibrinogen, fibronectin, vWF, vitronectin and thrombospondin. Next to this it is also a receptor for several soluble adhesive proteins.Vitronectin receptor is expressed on endothelial cells, some B cells, monocytes/macrophages, platelets and tumour cells. Vitronectin receptor binds next to vitronectin to fibrinogen, vWF, thrombospondin, fibronectin, osteopontin and collagen. Defects in human beta3 integrin are a cause of Glanzmann thrombasthenia, which is an autosomal recessive disorder characterized by mucocutaneous bleeding and the inability of this integrin to recognize macromolecular or synthetic peptide ligands. Aliases Platelet membrane glycoprotein IIIa, GPIIIa
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