For Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
Restrictions
For Research Use only
Buffer
PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.
The monoclonal antibody CF1 recognizes human peripheral myelin protein 22 (PMP22). PMP22 is a 160 amino acid glycoprotein and contains four hydrophobic domains, presumably transmembrane regions. It is the second most abundant protein in the mammalian nervous system, but is, at least in rodents, also found in other tissues, including the liver and gut. Myelin protein zero (P0) is the most abundant protein in mammalian peripheral nerve myelin. An interaction between P0 and PMP22 has been detected in compact myelin. Defects in PMP22 have been related to Charcot-Marie-Tooth disease (CMT) and inflammatory demyelinating neuropathy (IDP). Immunization with PMP22 induces experimental auto-immune neuritis without central nervous inflammation. Therefore, PMP22 is considered as candidate auto-antigen in inflammatory diseases of the peripheral nervous system, including both acute and chronic inflammatory demyelinating polyradiculoneuropathy. Monoclonal antibody CF1 is crossreactive with Rhesus monkey PMP22. Aliases Growth arrest-specific protein 3, Gas-3 Immunogen Peptide corresponding to amino acids 121-133 in the second extracellular domain of human PMP2 with an extra N-terminal cysteine residue (CRHPEWHLNSDYSYG)