Complement B + Bb antibody

Catalog No. ABIN2192095

Product Details

Target: Complement B + Bb

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunoassay (IA)

Sterility: 0.2 μm filtered

Clone: M13-12

Application Details

Application Notes: For Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.1 % bovine serum albumin and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References

Oppermann, Kurts, Zierz, Quentin, Weber, Götze: "Elevated plasma levels of the immunosuppressive complement fragment Ba in renal failure." in: Kidney international, Vol. 40, Issue 5, pp. 939-47, (1992) (PubMed).

Oppermann, Baumgarten, Brandt, Gottsleben, Kurts, Götze: "Quantitation of components of the alternative pathway of complement (APC) by enzyme-linked immunosorbent assays." in: Journal of immunological methods, Vol. 133, Issue 2, pp. 181-90, (1990) (PubMed).

Oppermann, Haubitz, Quentin, Götze: "Complement activation in patients with renal failure as detected through the quantitation of fragments of the complement proteins C3, C5, and factor B." in: Klinische Wochenschrift, Vol. 66, Issue 18, pp. 857-64, (1988) (PubMed).

Target Details

Target: Complement B + Bb

Background: The monoclonal antibody M13/12 recognizes human complement factor B/Bb. Factor B is an acute- phase protein. Levels of factor B increase during inflammation. Complement factor B is a single- chain molecule of 764 amino acids (MW of 90 kD), including a leader peptide of 25 amino acids. Factor B provides the catalytic subunit of the C3/C5 convertases of the alternative complement pathway. Assembly of the C3 convertase (C3bBb) requires binding of factor B to C3b (C3.H2O) and factor D- mediated cleavage of bound factor B resulting in the release of Ba (MW 33 kD). The C3 convertase is stabilized by the binding of properdin. This provides a positive amplification loop for the classical and alternative complement pathways. Bb (MW 60 kD) is the serine protease element of this convertase. After cleavage of C3, the C5 convertase ((C3b)2Bb) is formed. The Bb fragment may be regarded as a better indicator of the alternative pathway of complement activation than Ba as impaired renal filtration does directly influence Ba levels. Whereas Bb is elevated 2.2-fold in chronic renal failure (CRF) patients, plasma concentrations of Ba are 8.4-fold higher in CRF and 16-fold higher in end-stage renal disease (ESRD) patients compared to normals. In addition to complement activation, factor B fragments participate in other immunological functions.