Benzo[a]pyrenediol Epoxide antibody

Catalog No. ABIN2192144

Product Details anti-Benzo[a]pyrenediol Epoxide Antibody

Target: Benzo[a]pyrenediol Epoxide (BPDE)

Reactivity: Chemical

Host: Mouse

Clonality: Monoclonal

Conjugate: This Benzo[a]pyrenediol Epoxide antibody is un-conjugated

Application: Immunoassay (IA), Immunoprecipitation (IP)

Sterility: 0.2 μm filtered

Clone: 8E11

Application Details

Application Notes: The monoclonal antibody 8E11 can be used for immuno purification and for immuno assays as detector.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

References for anti-Benzo[a]pyrenediol Epoxide antibody (ABIN2192144)

Santella, Gammon, Zhang, Motykiewicz, Young, Hayes, Terry, Schoenberg, Brinton, Bose, Teitelbaum, Hibshoosh: "Immunohistochemical analysis of polycyclic aromatic hydrocarbon-DNA adducts in breast tumor tissue." in: Cancer letters, Vol. 154, Issue 2, pp. 143-9, (2000) (PubMed).

Mumford, Williams, Wilcosky, Everson, Young, Santella: "A sensitive color ELISA for detecting polycyclic aromatic hydrocarbon-DNA adducts in human tissues." in: Mutation research, Vol. 359, Issue 3, pp. 171-7, (1996) (PubMed).

Santella, Lin, Cleveland, Weinstein: "Monoclonal antibodies to DNA modified by a benzo[a]pyrene diol epoxide." in: Carcinogenesis, Vol. 5, Issue 3, pp. 373-7, (1984) (PubMed).

Target Details for Benzo[a]pyrenediol Epoxide

Target: Benzo[a]pyrenediol Epoxide (BPDE)

Abstract: BPDE Products

Target Type: Chemical

Background: A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representitive PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic. The monoclonal antibody 8E11 recognizes free BPDE and DNA adducts.