TNF alpha antibody

Catalog No. ABIN2192175

The Rabbit Polyclonal anti-TNF alpha antibody (ABIN2192175) specifically detects TNF alpha in WB, FACS, Neut, IA and ISt. The antibody is reactive with Human samples.

Quick Overview for TNF alpha antibody (ABIN2192175)

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TNF alpha antibody is un-conjugated

Application: Western Blotting (WB), Flow Cytometry (FACS), Neutralization (Neut), Immunoassay (IA), Immunostaining (ISt)

Product Details anti-TNF alpha Antibody

Sterility: 0.2 μm filtered

Application Details

Application Notes: For flow cytometry and Western blotting dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10. For neutralization of biological activity dilutions have to be made according to the amounts TNF-alpha to be inactivated. Before use in biological assays, the product must be filter sterilized and depending on the concentration to be used dialyzed against culture medium to remove the sodium azide added. Please inquire for availability of azide free solutions.

Restrictions: For Research Use only

Handling

Buffer: PBS, containing 0.02 % sodium azide and 0.1 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Product should be stored at 4 °C. Under recommended storage conditions, product is stable for one year.

Expiry Date: 12 months

Target Details for TNF alpha

Target: TNF alpha (Tumor Necrosis Factor alpha (TNF alpha))

Alternative Name: Tumor Necrosis Factor-alpha

Background: The antibody reacts with human natural and recombinant TNF-alpha as assessed by ELISA. The antibody inhibits the biological activity of human natural and recombinant TNF-alpha as determined with L929 and WEHI cells in a cytotoxicity assay. The antibody cross reacts with rhesus and cynomolgus natural TNF- alpha and lacks cross reactivity with human lymphotoxin.

Pathways: NF-kappaB Signaling, Apoptosis, Caspase Cascade in Apoptosis, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Inflammasome