eIF4EBP1 antibody (pThr70)
Quick Overview for eIF4EBP1 antibody (pThr70) (ABIN221236)
Target
See all eIF4EBP1 (EIF4EBP1) AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- pThr70
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Specificity
- Detects endogenous levels of 4E-BP1 only when phosphorylated at Thr70. Does not detect 4E-BP1 phosphorylated at other sites. Species cross-Reactivity: human, mouse, rat.
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Purification
- Protein A purified
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Immunogen
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Synthetic phosphopeptide derived from the sequence of mouse 4E-BP1.
Type of Immunogen: Synthetic phospho-peptide -
Isotype
- IgG
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Application Notes
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Approved: IP (1:50), WB (1:1000)
Usage: Suitable for use in Western Blot, and Immunoprecipitation. Western Blot: 1:1000 (Incubate membrane with diluted antibody in 5 % BSA, 1X TBS, 0.1 % Tween-20 at 4?C with gentle shaking.). Immunoprecipitation: 1:50. -
Comment
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Target Species of Antibody: Mouse
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA 50 % glycerol. No preservative added.
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Preservative
- Without preservative
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- eIF4EBP1 (EIF4EBP1) (Eukaryotic Translation Initiation Factor 4E Binding Protein 1 (EIF4EBP1))
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Alternative Name
- EIF4EBP1 / 4EBP1
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Background
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Name/Gene ID: EIF4EBP1
Synonyms: EIF4EBP1, 4E-BP1, 4EBP1, BP-1, PHAS-I, EIF4E-binding protein 1 -
Gene ID
- 1978
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UniProt
- Q13541
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Pathways
- MAPK Signaling, PI3K-Akt Signaling, RTK Signaling, AMPK Signaling, Regulation of Cell Size, BCR Signaling
Target
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