Vimentin antibody (FITC)

Catalog No. ABIN222825

The FITC-conjugated Mouse Monoclonal anti-Vimentin antibody (ABIN222825) specifically detects Vimentin in WB, IHC, ELISA, IF, IHC (p) and IHC (fro). The antibody is reactive with Human, Cow, Monkey, Chicken and Dog samples.

Quick Overview for Vimentin antibody (FITC) (ABIN222825)

Target: Vimentin (VIM)

Reactivity: Human, Cow, Monkey, Chicken, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Vimentin antibody is conjugated to FITC

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-Vimentin Antibody (FITC)

Specificity: Highly specific for the intermediate filament protein vimentin which is present in all cells of mesenchymal origin. This clone has turned out to be the most avid mab to vimentin. Polypeptide reacting: Mr 57000 intermediate filament protein (vimentin) of mesenchymal cells. Reactivities on Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. Species cross-reactivity: Human, monkey, bovine, canine, chicken, amphibia.

Purification: Protein A purified

Immunogen: Vimentin (purified from bovine lens).

Type of Immunogen: Purified protein

Isotype: IgG2a

Application Details

Application Notes: Approved: ELISA, IF, IHC, IHC-Fr, IHC-P, WB

Usage: Suitable for use in Western Blot, ELISA, Immunofluorescence microscopy and Immunohistochemistry. Immunohistochemistry (frozen and paraffin-embedded tissue and cytological material): 1:10 with PBS,  pH 7.4, Incubation Time 1 hr at RT, extended with paraffin. Protease pretreatment is required prior to antibody application. Positive control: Cultured RD cells, glioma cells, fibroblasts (SV-80), MDCK. Tumors specifically detected: sarcoma (including myosarcoma), lymphoma, melanoma. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Bovine

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Background: Name/Gene ID: VIM

Synonyms: VIM, Vimentin

Gene ID: 7431

UniProt: P08670

Pathways: Caspase Cascade in Apoptosis