Reticulon 4 antibody (3rd Transmembrane Domain)

Catalog No. ABIN224781

Product Details anti-Reticulon 4 Antibody

Target: Reticulon 4 (RTN4)

Binding Specificity: 3rd Transmembrane Domain

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Reticulon 4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Recognizes human Neurite Outgrowth Inhibitor Protein ABC (NogoABC). The immunogen peptide is within the 25 amino acid region identified for the inhibition of neurite outgrowth. No significant sequence homology with other known proteins. Species cross-reactivity: Human. Species sequence Homology: rat: 92 % Nogo or rat foocen-m1.2 protein.

Purification: Immunoaffinity purified

Immunogen: Synthetic peptide consisting of 13aa between the putative 2nd-3rd transmembrane domain of human NogoA (KLH coupled).

Type of Immunogen: Synthetic peptide

Isotype: IgG

Application Details

Application Notes: Approved: ELISA (1:10000 - 1:100000), WB (1 - 10 μg/mL)

Usage: Suitable for use in Western Blot and ELISA. Western Blot (ECL): 1-10 μg/mL. Calculated MW: ~135kD. Reported MW for full length NogoA: ~180kD. ELISA: 1:10000-1:100000. Using 50-100 ng control peptide per well.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.1 % BSA, 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C,-20 °C

Storage Comment: 4°C or -20°C, Avoid freeze-thaw cycles.

Target Details for Reticulon 4

Target: Reticulon 4 (RTN4)

Alternative Name: RTN4 / Nogo (RTN4 Products)

Synonyms: ASY antibody, NI220/250 antibody, NOGO antibody, NOGO-A antibody, NOGOC antibody, NSP antibody, NSP-CL antibody, Nbla00271 antibody, Nbla10545 antibody, Nogo-B antibody, Nogo-C antibody, RTN-X antibody, RTN4-A antibody, RTN4-B1 antibody, RTN4-B2 antibody, RTN4-C antibody, RTN4-Cw antibody, DKFZp459C0314 antibody, 1110020G17Rik antibody, AA407876 antibody, AA409940 antibody, AA960376 antibody, C130026I10Rik antibody, NgA antibody, Nogo-A antibody, mKIAA0886 antibody, mKIAA4153 antibody, NI-250 antibody, Nogo antibody, Vp20 antibody, r antibody, rat N antibody, rat NogoA antibody, reticulon-4 antibody, nogo antibody, rtn4 antibody, wu:fb17a02 antibody, wu:fb22f12 antibody, wu:fd61a07 antibody, zgc:92163 antibody, reticulon 4 antibody, reticulon 4a antibody, RTN4 antibody, rtn4 antibody, Rtn4 antibody, rtn4a antibody

Background: Name/Gene ID: RTN4
Family: Reticulon

Synonyms: RTN4, Human NogoA, My043 protein, Neurite outgrowth inhibitor, NOGOC, NSP-CL, Nogo protein, KIAA0886, Reticulon 5, Reticulon-4, Reticulon-5, Reticulon 4, RTN4-B1, RTN-X, RTN4-A, RTN4-B2, RTN4-C, Nbla00271, Neurite growth inhibitor 220, NI220/250, NOGO-A, Nogo-C, ASY, Foocen, Nbla10545, NOGO, Nogo-B, NSP

Gene ID: 57142

UniProt: Q9NQC3

Pathways: Neurotrophin Signaling Pathway, Regulation of Cell Size, SARS-CoV-2 Protein Interactome