MLANA antibody
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- Target See all MLANA Antibodies
- MLANA (Melan A (MLANA))
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Reactivity
- Human, Horse
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This MLANA antibody is un-conjugated
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Application
- Western Blotting (WB), Flow Cytometry (FACS), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP)
- Specificity
- Recognizes MART-1 at 18kD. Labels melanomas and other tumors showing melanocytic differentiation. Does not label tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. Species cross-reactivity: Human and equine. Does not cross-react with mouse or rat.
- Purification
- Protein A purified
- Immunogen
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Recombinant hMART-1 protein. MW: 18kD. Cellular Localization: Cytoplasmic.
Type of Immunogen: Recombinant protein - Isotype
- IgG2b kappa
- Top Product
- Discover our top product MLANA Primary Antibody
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- Application Notes
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Approved: Flo, IHC, IHC-P, IP, WB (0.5 - 1 μg/mL)
Usage: Suitable for use in Flow Cytometry, Immunoprecipitation, Western Blot and Immunohistochemistry. Immunoprecipitation (Denatured verified): 2 μg/mg protein lysate. (Use Protein A). Western Blot: 0.5-1 μg/mL for 2 hours at RT. Immunohistochemistry (formalin/paraffin): 1-2 μg/mL for 30 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Positive control: CaC1 melanoma cells and melanomas. - Comment
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Target Species of Antibody: Human
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- PBS, pH 7.4, 0.1 % BSA, 0.05 % sodium azide, 40 % glycerol.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeat freeze-thaw cycles.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Target
- MLANA (Melan A (MLANA))
- Alternative Name
- MLANA / Melan-A / MART-1 (MLANA Products)
- Synonyms
- A930034P04Rik antibody, Mart1 antibody, MART-1 antibody, MART1 antibody, melan-A antibody, Mlana antibody, MLANA antibody
- Background
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Name/Gene ID: MLANA
Synonyms: MLANA, Antigen LB39-AA, Antigen SK29-AA, Melan-A, Protein Melan-A, MART-1, MART1 - Gene ID
- 2315
- UniProt
- Q16655
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