RAF1 antibody (pSer259)

Catalog No. ABIN233710

This Rabbit Polyclonal antibody specifically detects RAF1 in WB and IP. It exhibits reactivity toward Human, Rat, Chicken and Xenopus laevis.

Quick Overview for RAF1 antibody (pSer259) (ABIN233710)

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Reactivity: Human, Rat, Chicken, Xenopus laevis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAF1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Product Details anti-RAF1 Antibody

Binding Specificity: pSer259

Specificity: Detects endogenous levels of c-Raf only when phosphorylated at Ser259. Recognizes rat, chicken, Xenopus.

Purification: Protein A purified

Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser259 of human c-Raf.

Type of Immunogen: Synthetic phospho-peptide

Isotype: IgG

Application Details

Application Notes: Approved: IP (1:50), WB (1:1000)

Usage: Suitable for use in Western Blot and Immunoprecipitation. Western Blot: 1:1000. Immunoprecipitation: 1:50.

Comment:

Target Species of Antibody: Human

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: 10 mM HEPES,  pH 7.5, 150 mM sodium chloride, 0.1 mg/mL BSA, 50 % glycerol. No preservative added.

Preservative: Without preservative

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for RAF1

Target: RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))

Alternative Name: RAF1 / RAF

Background: Name/Gene ID: RAF1
Subfamily: Raf
Family: Protein Kinase

Synonyms: RAF1, C-Raf, C-raf-1, CRAF, NS5, Raf-1, Proto-oncogene c-RAF, Oncogene RAF1, RAF, Raf1 kinase

Gene ID: 5894

UniProt: P04049

Pathways: MAPK Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, cAMP Metabolic Process, Stem Cell Maintenance, Hepatitis C, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling, BCR Signaling