CD13 Myeloid Cell antibody (Biotin)
Reactivity: Human
FACS, IHC (f)
Host: Mouse
Monoclonal
22A5
Biotin
-
- Target
- CD13 Myeloid Cell
- Reactivity
- Human
-
Host
- Mouse
-
Clonality
- Monoclonal
-
Conjugate
- Biotin
-
Application
- Flow Cytometry (FACS), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))
- Specificity
- Recognizes the (Mr 150-170 kDa) cell surface glycoprotein expressed in a pan-myeloid fashion. This antibody also reacts with osteoclasts in giant cell tumors of bone (osteoclastoma), clear cell chondrosarcoma and aneurysmal bone cysts (1). The CD13 antigen is present on most cells of myeloid origin, including granulocytes and monocytes in normal peripheral blood. CD13 is not expressed on B-cells, T-cells, platelets or erythrocytes. Expression of this antigen is greater on monocytes than on granulocytes.
- Characteristics
- MAb to CD13 Myeloid Cells, Monoclonal antibody to Human CD13 Myeloid cells Biotin conjugated
- Purification
- Protein A Chromatography. Tissue culture
- Immunogen
- A cell suspension containing osteoclasts from osteoclastomas
- Clone
- 22A5
- Isotype
- IgG
-
-
- Application Notes
- Suitable for use in flow cytometry and immunohistochemistry (acetone-fixed frozen sections). We recommend using 1 µg to stain 1.0 x 106 cells in flow cytometric applications. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
- Restrictions
- For Research Use only
-
- Format
- Liquid
- Buffer
- 0.01 M PBS, pH 7.2 containing 2 mM EDTA, 1 % BSA, 0.1 % Sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
-
DO NOT FREEZE.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use. - Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
-
- Target
- CD13 Myeloid Cell
- Alternative Name
- CD13 (Myeloid cells)
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