Shigatoxin (STX) 1 & 2 antibody
Reactivity: E. coli
Neut, ELISA, WB
Host: Mouse
Monoclonal
Mixed
unconjugated
-
- Target
- Shigatoxin (STX) 1 & 2
- Reactivity
- E. coli
- Host
- Mouse
- Clonality
- Monoclonal
- Application
- Neutralization (Neut), ELISA, Western Blotting (WB)
- Specificity
- Reacts with Escherichia coli (E. coli) Shigatoxin (STX) 1 & 2
- Characteristics
- MAb to E. coli STX 1 & 2, Monoclonal antibody to Escherichia coli (E. coli) Shigatoxin (STX) 1 & 2, pooled IgG
- Purification
- Protein A Chromatography. Ascites
- Clone
- Mixed
- Isotype
- IgG
-
- Application Notes
- Suitable for use in ELISA (capture antibody at 20- g/mL), Western blot (1- g/mL) and Toxin Neutralization. Neutralizes Vero Cell toxicity of E. coli O157:H7 extracts when used at a 5 µg/mL concentration. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.
- Comment
-
Concentration (detection method): 1 mg/mL (prior to lyophilization)
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Reconstitute with 1 mL deionized water.
- Buffer
- Lyophilized from 5 mM Phosphate buffer, pH 7.2 - 7.4.
- Preservative
- Without preservative
- Handling Advice
-
Avoid repeated freeze/thaw cycles.
Centrifuge product if not completely clear after standing at room temperature.
Prepare working dilution only prior to immediate use. - Storage
- 4 °C/-20 °C
- Storage Comment
- Store lyophilized product at 2-8 °C. After reconstitution, short term (up to 2 weeks) store at 2-8 °C. Long term (up to one year) aliquot and store at -28 °C. If aliquoted for long term storage, fill volume should be equal to or greater than 50 % of the nominal fill volume of the vial used.
- Expiry Date
- 12 months
-
- Target
- Shigatoxin (STX) 1 & 2
-
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