Streptavidin antibody

Catalog No. ABIN236393

Product Details anti-Streptavidin Antibody

Target: Streptavidin

Reactivity: Streptomyces avidinii

Host: Mouse

Clonality: Monoclonal

Conjugate: This Streptavidin antibody is un-conjugated

Application: ELISA

Cross-Reactivity (Details): Not cross-reactive with egg white avidin. Binds with high efficiency to epitope of streptavidin molecule. K86130M reacts with the determinant not overlapping with K86310M epitope. Binds to the streptavidin without interfering with the biotin-binding reaction.

Characteristics: MAb to Streptavidin, Monoclonal antibody to Streptavidin

Purification: Protein G Chromatography. Ascites

Purity: > 95 % pure

Clone: S8C12

Isotype: IgG1

Application Details

Application Notes: Can be used to localize streptavidin, to amplify signals in immunochemistry, histochemistry, ELISA, flow cytometry and immunoblotting. Mouse anti-streptavidin applied to the ABC complex, previously bound to the second antibody system, allows for further enhancement of signal when anti-mouse antibody conjugate is added. The antibody is effective in Immunoadsorbtion procedures of streptavidin-biotinylated protein complexes. Each laboratory should determine an optimum working titer for use in its particular application. Other applications have not been tested but use in such assays should not necessarily be excluded.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: PBS, pH 7.4, 0.1 % Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for Streptavidin

Target: Streptavidin

Abstract: Streptavidin Products