HLA-E antibody

Catalog No. ABIN238482

The Mouse Monoclonal anti-HLA-E antibody (Clone MEM-E-07) (ABIN238482) specifically detects HLA-E in FACS, IP and IHC (p). The antibody is reactive with Human samples.

Quick Overview for HLA-E antibody (ABIN238482)

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HLA-E antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: MEM-E-07

Product Details anti-HLA-E Antibody

Specificity: The antibody MEM-E/07 recognized native surface-expressed HLA-E, but not denaturated heavy chain of HLA-E. The published results revealed that the antibody cross-reacts with some classical MHC Class I molecules (MHC Class Ia): HLA-B7 (strongly), HLA-B8 (moderately), HLA-B27, -B44 (weakly).

Characteristics: Synonyms: HLA-6.2, HLAE, MHC class I antigen E, HLA class I histocompatibility antigen, alpha chain E

Purification: Protein A affinity chromatography

Purity: > 95 % by SDS-PAGE

Immunogen: Bacterially expressed recombinant HLA-E refolded with beta2-microglobulin and peptideRemarks: Gene Locus: 6p21. 3

Isotype: IgG1

Application Details

Application Notes: Flow cytometry. Immunoprecipitation. Immunohistochemistry on paraffin sections: 10 mg/mL, positive tissue: spleen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoidrepeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for HLA-E

Target: HLA-E (HLA Class I Histocompatibility Antigen, alpha Chain E (HLA-E))

Background: HLA-E belongs to the MHC Class I molecules (MHC Class Ib, nonclassical) and it is expressed on many types of the human cells.Synonyms: HLA class I histocompatibility antigen, HLA-6.2, HLAE, MHC class I antigen E, alpha chain E

Gene ID: 3133

UniProt: P13747

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Human Leukocyte Antigen (HLA) in Adaptive Immune Response