GFP antibody

Catalog No. ABIN243767

The Rabbit Polyclonal anti-GFP antibody is suitable to detect GFP in samples from Aequorea victoria. It has been validated for WB, IHC, IF, IP, ICC and IHC (p).

Quick Overview for GFP antibody (ABIN243767)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-GFP Antibody

Specificity: Recognizes Green Fluorescent Protein (GFP). Species cross-reactivity: all variants of Aequorea victoria GFP, S65T-GFP, RS-GFP, YFP and EGFP. Detects 5 ng of recombinant GFP (ECL) with 1 minute exposure or less.

Purification: Affinity purified

Immunogen: Highly purified recombinant GFP made in E. coli.

Isotype: IgG

Application Details

Application Notes: Approved: ICC (1:200 - 1:1000), IF, IHC, IHC-P (1:2500 - 1:10000), IP, WB (1:2500 - 1:10000)

Usage: Suitable for use in Western Blot, Immunoprecipitation and Immunocytochemistry, Immunohistochemistry, Immunofluorescence and Immunogold Electron Microscopy. Western Blot: 1:2500-1:10000 in 1X BLOTTO or 3 % BSA in PBS. Detects the GFP fraction from cell extracts expressing recombinant GFP fusion proteins. Immunocytochemistry: 1:200-1:1000 in 4 % normal serum. Very good signal on recombinant YES-GFP chimeras expressed in COS cells. Immunohistochemistry: 1:200 with HRP detection systems. Has been shown to be useful on mouse sections fixed with formalin. Immunohistochemistry: 1:200 for paraffin sections. Requires heat mediated antigen retrieval via the microwave method before IHC staining (13 min in 10 mM citrate buffer  pH 6 followed by rapid cooling on ice). Immunoprecipitation: 1 μL per 10cm tissue culture dish of transfected cells. Use 10 μL protein A agarose CL4B to precipitate immune complex. Immunogold Electron Microscopy: 1:100.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212