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GFP antibody (Biotin)

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB, ELISA, IHC and IF. Suitable for Aequorea victoria.
Catalog No. ABIN243774
$1,369.13
Plus shipping costs $50.00
500 μg
Shipping to: United States
Delivery in 11 to 14 Business Days

Quick Overview for GFP antibody (Biotin) (ABIN243774)

Target

See all GFP Antibodies
GFP (Green Fluorescent Protein (GFP))

Reactivity

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Aequorea victoria

Host

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Mouse

Clonality

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Monoclonal

Conjugate

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This GFP antibody is conjugated to Biotin

Application

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Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone

1002
  • Specificity

    Recognizes wild type and recombinant forms of Green Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-mouse serum. Does not recognize enhanced forms of GFP.

    Purification

    Protein A purified

    Immunogen

    GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

    Type of Immunogen: Fusion protein

    Isotype

    IgG1 kappa
  • Application Notes

    Approved: ELISA (1:10000), IF, IHC (1:1000 - 1:3000), WB (1:1000 - 1:5000)

    Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000 (sandwich or capture). Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:1000-1:3000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    Comment

    Target Species of Antibody: Aequorea victoria

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Handling Advice

    Avoid repeat freeze-thaw cycles.

    Storage

    4 °C,-20 °C

    Storage Comment

    Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
  • Target

    GFP (Green Fluorescent Protein (GFP))

    Alternative Name

    GFP

    Background

    Name/Gene ID: GFP

    Synonyms: GFP

    UniProt

    P42212
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