GFP antibody (Biotin)

Catalog No. ABIN243774

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB, ELISA, IHC and IF. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (Biotin) (ABIN243774)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is conjugated to Biotin

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 1002

Product Details anti-GFP Antibody (Biotin)

Specificity: Recognizes wild type and recombinant forms of Green Fluorescent Protein (GFP). Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin and anti-mouse serum. Does not recognize enhanced forms of GFP.

Purification: Protein A purified

Immunogen: GST-Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

Type of Immunogen: Fusion protein

Isotype: IgG1 kappa

Application Details

Application Notes: Approved: ELISA (1:10000), IF, IHC (1:1000 - 1:3000), WB (1:1000 - 1:5000)

Usage: Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry. ELISA: 1:10000 (sandwich or capture). Western Blot: 1:1000-1:5000. Immunohistochemistry: 1:1000-1:3000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Comment:

Target Species of Antibody: Aequorea victoria

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS,  pH 7.2, 10 mg/mL BSA (IgG, protease free), 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeat freeze-thaw cycles.

Storage: 4 °C,-20 °C

Storage Comment: Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: Name/Gene ID: GFP

Synonyms: GFP

UniProt: P42212