NEK3 antibody

Catalog No. ABIN2459259

Product Details anti-NEK3 Antibody

Target: NEK3 (NIMA related kinase 3 (NEK3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NEK3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NEK3.

Application Details

Application Notes: NEK3 antibody can be used for detection of NEK3 by ELISA at 1:1562500. NEK3 antibody can be used for detection of NEK3 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store NEK3 antibody at -20 °C.

Target Details for NEK3

Target: NEK3 (NIMA related kinase 3 (NEK3))

Alternative Name: NEK3 (NEK3 Products)

Synonyms: HSPK36 antibody, ATNEK3 antibody, NIMA-RELATED KINASE3 antibody, NIMA-related kinase 3 antibody, T8M17.60 antibody, T8M17_60 antibody, NIMA related kinase 3 antibody, NIMA (never in mitosis gene a)-related expressed kinase 3 antibody, NIMA-related kinase 3 S homeolog antibody, NIMA-related kinase 3 antibody, NEK3 antibody, Nek3 antibody, nek3.S antibody

Background: NEK3 is a member of the NimA (never in mitosis A) family of serine/threonine protein kinases. It differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. The kinase is activated by prolactin stimulation, leading to phosphorylation of VAV2 guanine nucleotide exchange factor, paxillin, and activation of the RAC1 GTPase.In Aspergillus nidulans, lack of the serine/threonine kinase NimA (never in mitosis A) results in cell cycle arrest in G2, while overexpression causes the premature onset of mitotic events. The protein encoded by this gene is similar in sequence to the Aspergillus nidulans protein and may therefore play a role in mitotic regulation. However, the encoded protein differs from other NimA family members in that it is not cell cycle regulated and is found primarily in the cytoplasm. Three transcript variants have been found for this gene, but the full-length nature of only two of them has been characterized.

Molecular Weight: 58 kDa

Gene ID: 4752

NCBI Accession: NP_002489

UniProt: P51956