CAPN10 antibody

Catalog No. ABIN2459529

Product Details anti-CAPN10 Antibody

Target: CAPN10 (Calpain 10 (CAPN10))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CAPN10 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CAPN10.

Application Details

Application Notes: CAPN10 antibody can be used for detection of CAPN10 by ELISA at 1:1562500. CAPN10 antibody can be used for detection of CAPN10 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store CAPN10 antibody at -20 °C.

Target Details for CAPN10

Target: CAPN10 (Calpain 10 (CAPN10))

Alternative Name: CAPN10 (CAPN10 Products)

Synonyms: CAPN10 antibody, CANP10 antibody, NIDDM1 antibody, AW049679 antibody, Capn8 antibody, mKIAA1845 antibody, calpain 10 antibody, calpain 10 L homeolog antibody, CAPN10 antibody, capn10.L antibody, capn10 antibody, Capn10 antibody

Background: CAPN10 is the calcium-regulated non-lysosomal thiol-protease which catalyzes limited proteolysis of substrates involved in cytoskeletal remodeling and signal transduction.Calpains are ubiquitous, well-conserved family of calcium-dependent, cysteine proteases. The calpain proteins are heterodimers consisting of an invariant small subunit and variable large subunits. The large catalytic subunit has four domains: domain I, the N-terminal regulatory domain that is processed upon calpain activation, domain II, the protease domain, domain III, a linker domain of unknown function, and domain IV, the calmodulin-like calcium-binding domain. This gene encodes a large subunit. It is an atypical calpain in that it lacks the calmodulin-like calcium-binding domain and instead has a divergent C-terminal domain. It is similar in organization to calpains 5 and 6. This gene is associated with type 2 or non-insulin-dependent diabetes mellitus (NIDDM) and located within the NIDDM1 region. Multiple alternative transcript variants encoding different isoforms have been described for this gene.

Molecular Weight: 58 kDa

Gene ID: 11132

NCBI Accession: NP_075573

UniProt: Q9HC96

Pathways: Positive Regulation of Peptide Hormone Secretion