GLE1 antibody

Catalog No. ABIN2459776

Product Details anti-GLE1 Antibody

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GLE1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human GLE1.

Application Details

Application Notes: GLE1 antibody can be used for detection of GLE1 by ELISA at 1:1562500. GLE1 antibody can be used for detection of GLE1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store GLE1 antibody at -20 °C.

Target Details for GLE1

Target: GLE1 (GLE1 RNA Export Mediator (GLE1))

Alternative Name: GLE1 (GLE1 Products)

Synonyms: Dmel\\CG14749 antibody, GLE1 antibody, GLE1L antibody, LCCS antibody, LCCS1 antibody, hGLE1 antibody, Gle1l antibody, 4933405K21Rik antibody, AA553313 antibody, CG14749 gene product from transcript CG14749-RA antibody, nucleoporin GLE1 antibody, RNA export factor Gle1 antibody, GLE1, RNA export mediator antibody, GLE1 RNA export mediator antibody, GLE1 RNA export mediator (yeast) antibody, CG14749 antibody, CpipJ_CPIJ014147 antibody, SJAG_03847 antibody, GLE1 antibody, Gle1 antibody

Background: GLE1 is a predicted 75- kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly (A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly (A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm.This gene encodes a predicted 75- kDa polypeptide with high sequence and structure homology to yeast Gle1p, which is nuclear protein with a leucine-rich nuclear export sequence essential for poly (A)+RNA export. Inhibition of human GLE1L by microinjection of antibodies against GLE1L in HeLa cells resulted in inhibition of poly (A)+RNA export. Immunoflourescence studies show that GLE1L is localized at the nuclear pore complexes. This localization suggests that GLE1L may act at a terminal step in the export of mature RNA messages to the cytoplasm. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 75 kDa

Gene ID: 2733

NCBI Accession: NP_001490

UniProt: Q53GS7