NMI antibody can be used for detection of NMI by ELISA at 1:62500. NMI antibody can be used for detection of NMI by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Restrictions
For Research Use only
Format
Lyophilized
Reconstitution
Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
Concentration
1 mg/mL
Buffer
Antibody is lyophilized in PBS buffer with 2 % sucrose.
Handling Advice
As with any antibody avoid repeat freeze-thaw cycles.
Storage
4 °C/-20 °C
Storage Comment
For short periods of storage (days) store at 4 °C. For longer periods of storage, store NMI antibody at -20 °C.
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Background
NMYC interactor (NMI) encodes a protein that interacts with NMYC and CMYC (two members of the oncogene Myc family), and other transcription factors containing a Zip, HLH, or HLH-Zip motif. The NMI protein also interacts with all STATs except STAT2 and augments STAT-mediated transcription in response to cytokines IL2 and IFN-gamma. The NMI mRNA has high expression in myeloid leukemia cell lines.