MCM4 antibody

Catalog No. ABIN2461392

The Rabbit Polyclonal anti-MCM4 antibody (ABIN2461392) specifically detects MCM4 in WB and ELISA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for MCM4 antibody (ABIN2461392)

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MCM4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MCM4 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human MCM4.

Application Details

Application Notes: MCM4 antibody can be used for detection of MCM4 by ELISA at 1:12500. MCM4 antibody can be used for detection of MCM4 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store MCM4 antibody at -20 °C.

Target Details for MCM4

Target: MCM4 (Minichromosome Maintenance Deficient 4 (MCM4))

Alternative Name: MCM4

Background: The protein encoded by MCM4 is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 6 and 7 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. The phosphorylation of this protein by CDC2 kinase reduces the DNA helicase activity and chromatin binding of the MCM complex. The MCM4 gene is mapped to a region on the chromosome 8 head-to-head next to the PRKDC/DNA-PK, a DNA-activated protein kinase involved in the repair of DNA double-strand breaks.

Molecular Weight: 95 kDa, 97 kDa

Gene ID: 4173

NCBI Accession: NP_005905

UniProt: P33991

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA