RUNX2 antibody

Catalog No. ABIN2461404

The Rabbit Polyclonal anti-RUNX2 antibody (ABIN2461404) specifically detects RUNX2 in WB and ELISA. The antibody is reactive with Human, Mouse, Rat and Dog samples.

Quick Overview for RUNX2 antibody (ABIN2461404)

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RUNX2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-RUNX2 Antibody

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RUNX2.

Application Details

Application Notes: RUNX2 antibody can be used for detection of RUNX2 by ELISA at 1:1562500. RUNX2 antibody can be used for detection of RUNX2 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RUNX2 antibody at -20 °C.

Target Details for RUNX2

Target: RUNX2 (Runt-Related Transcription Factor 2 (RUNX2))

Alternative Name: RUNX2

Background: RUNX2 is a member of the RUNX family of transcription factors and encodes a nuclear protein with an Runt DNA-binding domain. This protein is essential for osteoblastic differentiation and skeletal morphogenesis, acting as a scaffold for nucleic acids and regulatory factors involved in skeletal gene expression. The protein can bind DNA both as a monomer or, with more affinity, as a subunit of a heterodimeric complex. Mutations in this gene have been associated with the bone development disorder cleidocranial dysplasia (CCD). Transcript variants, encoding different protein isoforms, result from alternate promoter use as well as alternate splicing.

Molecular Weight: 57 kDa, 55 kDa

Gene ID: 860

NCBI Accession: NP_001019801

UniProt: Q13950