ENO1 antibody

Catalog No. ABIN2461629

Product Details anti-ENO1 Antibody

Target: ENO1 (Enolase 1 (ENO1))

Reactivity: Human, Zebrafish (Danio rerio), Arabidopsis, C. elegans

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ENO1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ENO1.

Application Details

Application Notes: ENO1 antibody can be used for detection of ENO1 by ELISA at 1:62500. ENO1 antibody can be used for detection of ENO1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ENO1 antibody at -20 °C.

Target Details for ENO1

Target: ENO1 (Enolase 1 (ENO1))

Alternative Name: ENO1 (ENO1 Products)

Synonyms: enolase 1 antibody, F2P9.10 antibody, F2P9_10 antibody, Pgh1 antibody, pENO1 antibody, enolase 1 antibody, phosphopyruvate hydratase antibody, ENO1 antibody, eno1 antibody

Background: ENO1 is one of three enolase isoenzymes found in mammals, its gene encodes alpha-enolase, a homodimeric soluble enzyme, and also encodes a shorter monomeric structural lens protein, tau-crystallin. The two proteins are made from the same message. The full length protein, the isoenzyme, is found in the cytoplasm. The shorter protein is produced from an alternative translation start, is localized to the nucleus, and has been found to bind to an element in the c-myc promoter. A pseudogene has been identified that is located on the other arm of the same chromosome.

Molecular Weight: 47 kDa

Gene ID: 2023

NCBI Accession: NP_001419

UniProt: P06733