RNF8 antibody

Catalog No. ABIN2462018

Product Details anti-RNF8 Antibody

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RNF8 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human RNF8.

Application Details

Application Notes: RNF8 antibody can be used for detection of RNF8 by ELISA at 1:312500. RNF8 antibody can be used for detection of RNF8 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store RNF8 antibody at -20 °C.

Target Details for RNF8

Target: RNF8 (Ring Finger Protein 8 (RNF8))

Alternative Name: RNF8 (RNF8 Products)

Synonyms: hRNF8 antibody, 3830404E21Rik antibody, AIP37 antibody, RNF8 antibody, rnf8 antibody, fc66f09 antibody, wu:fc66f09 antibody, zgc:55936 antibody, zgc:77313 antibody, ring finger protein 8 antibody, ring finger protein 8, E3 ubiquitin protein ligase S homeolog antibody, ring finger protein 8, E3 ubiquitin protein ligase L homeolog antibody, ring finger protein 8, E3 ubiquitin protein ligase antibody, RNF8 antibody, Rnf8 antibody, rnf8.S antibody, rnf8.L antibody, rnf8 antibody

Background: RNF8 contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins.The protein encoded by this gene contains a RING finger motif and a FHA domain. This protein has been shown to interact with several class II ubiquitin-conjugating enzymes (E2), including UBE2E1/UBCH6, UBE2E2, and UBE2E3, and may act as an ubiquitin ligase (E3) in the ubiquitination of certain nuclear proteins. Alternatively spliced transcript variants encoding distinct isoforms have been reported.

Molecular Weight: 55 kDa, 51 kDa

Gene ID: 9025

NCBI Accession: NP_003949

UniProt: O76064

Pathways: Production of Molecular Mediator of Immune Response