PUF60 antibody
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- Target See all PUF60 Antibodies
- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This PUF60 antibody is un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Purification
- Antibody is purified by protein A chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PUF60.
- Top Product
- Discover our top product PUF60 Primary Antibody
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- Application Notes
- PUF60 antibody can be used for detection of PUF60 by ELISA at 1:312500. PUF60 antibody can be used for detection of PUF60 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store PUF60 antibody at -20 °C.
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- Target
- PUF60 (Poly-U Binding Splicing Factor 60KDa (PUF60))
- Alternative Name
- PUF60 (PUF60 Products)
- Synonyms
- FIR antibody, RoBPI antibody, SIAHBP1 antibody, fc21a05 antibody, wu:fc21a05 antibody, wu:fi43a08 antibody, 2410104I19Rik antibody, 2810454F19Rik antibody, Siahbp1 antibody, fe37c05 antibody, repressor antibody, si:ch211-12p8.2 antibody, si:zc12p8.2 antibody, wu:fb33e11 antibody, wu:fe37c05 antibody, zgc:86806 antibody, poly(U) binding splicing factor 60 antibody, poly-U binding splicing factor a antibody, poly-U binding splicing factor 60 antibody, poly-U binding splicing factor b antibody, PUF60 antibody, puf60a antibody, Puf60 antibody, puf60b antibody
- Background
- PUF60 is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription.The protein encoded by this gene is a Ro RNP-binding protein. It interacts with Ro RNPs and their interaction is thought to represent a gain of function for Ro RNPs. This protein also forms a ternary complex with far upstream element (FUSE) and FUSE-binding protein. It can repress a c-myc reporter via the FUSE. It is also known to target transcription factor IIH and inhibit activated transcription. This gene is implicated in the xeroderma pigmentosum disorder. There are two alternatively spliced transcript variants of this gene encoding different isoforms. There seems to be evidence of multiple polyadenylation sites for this gene.
- Molecular Weight
- 58 kDa
- Gene ID
- 22827
- NCBI Accession
- NP_055096
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