Argininosuccinate synthetase antibody
Reactivity: Human, Dog, Mouse, Rat
IHC, ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
1 image
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- Target
- Argininosuccinate synthetase
- Reactivity
- Human, Dog, Mouse, Rat
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
- Purification
- Antibody is purified by protein A chromatography method.
- Immunogen
- Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ASS.
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- Application Notes
- ASS antibody can be used for detection of ASS by ELISA at 1:312500. ASS antibody can be used for detection of ASS by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.
- Concentration
- 1 mg/mL
- Buffer
- Antibody is lyophilized in PBS buffer with 2 % sucrose.
- Handling Advice
- As with any antibody avoid repeat freeze-thaw cycles.
- Storage
- 4 °C/-20 °C
- Storage Comment
- For short periods of storage (days) store at 4 °C. For longer periods of storage, store ASS antibody at -20 °C.
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- Target
- Argininosuccinate synthetase
- Background
- ASS catalyzes the penultimate step of the arginine biosynthetic pathway.The protein encoded by this gene catalyzes the penultimate step of the arginine biosynthetic pathway. There are approximately 10 to 14 copies of this gene including the pseudogenes scattered across the human genome, among which the one located on chromosome 9 appears to be the only functional gene for argininosuccinate synthetase. Mutations in the chromosome 9 copy of ASS cause citrullinemia. Two transcript variants encoding the same protein have been found for this gene.
- Molecular Weight
- 45 kDa, 47 kDa
- Gene ID
- 445
- NCBI Accession
- NP_446464
- UniProt
- P00966
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