PRP19 antibody

Catalog No. ABIN2462670

Product Details anti-PRP19 Antibody

Target: PRP19 (PRPF19) (Pre-mRNA Processing Factor 19 (PRPF19))

Reactivity: Human, Mouse, Rat, Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRP19 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by protein A chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRPF19.

Application Details

Application Notes: PRPF19 antibody can be used for detection of PRPF19 by ELISA at 1:312500. PRPF19 antibody can be used for detection of PRPF19 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 100 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store PRPF19 antibody at -20 °C.

Target Details for PRP19

Target: PRP19 (PRPF19) (Pre-mRNA Processing Factor 19 (PRPF19))

Alternative Name: PRPF19 (PRPF19 Products)

Synonyms: NMP200 antibody, fb18f09 antibody, zgc:56158 antibody, wu:fb18f09 antibody, nmp200 antibody, PRP19 antibody, PSO4 antibody, SNEV antibody, UBOX4 antibody, hPSO4 antibody, Prp19 antibody, nmp-200 antibody, AA617263 antibody, AL024362 antibody, D19Wsu55e antibody, Snev antibody, pre-mRNA processing factor 19 antibody, pre-mRNA processing factor 19 L homeolog antibody, prpf19 antibody, prpf19.L antibody, PRPF19 antibody, Prpf19 antibody

Background: PRPF19 plays a role in DNA double-strand break (DSB) repair and pre-mRNA splicing reaction. It binds double-stranded DNA in a sequence-nonspecific manner. PRPF19 acts as a structural component of the nuclear framework. It may also serve as a support for spliceosome binding and activity. It is essential for spliceosome assembly in a oligomerization-dependent manner and might also be important for spliceosome stability. It also may have E3 ubiquitin ligase activity. The PSO4 complex is required in the DNA interstrand cross-links (ICLs) repair process. Overexpression of PRPF19 might extend the cellular life span by increasing the resistance to stress or by improving the DNA repair capacity of the cells.In S. cerevisiae, Pso4 has pleiotropic functions in DNA recombination and in error-prone nonhomologous end-joining DNA repair.

Molecular Weight: 55 kDa

Gene ID: 27339

NCBI Accession: NP_055317

UniProt: Q9UMS4

Pathways: Ribonucleoprotein Complex Subunit Organization