ZP4 antibody

Catalog No. ABIN2463282

Product Details anti-ZP4 Antibody

Target: ZP4 (Zona Pellucida Glycoprotein 4 (ZP4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZP4 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human ZP4.

Application Details

Application Notes: ZP4 antibody can be used for detection of ZP4 by ELISA at 1:1562500. ZP4 antibody can be used for detection of ZP4 by western blot at 0.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store ZP4 antibody at -20 °C.

Target Details for ZP4

Target: ZP4 (Zona Pellucida Glycoprotein 4 (ZP4))

Alternative Name: ZP4 (ZP4 Products)

Synonyms: ZPB antibody, xlZPB antibody, lzpb-a antibody, MGC154827 antibody, zbp antibody, zp1 antibody, ZBP antibody, TAC4 antibody, MGC154888 antibody, ZP1 antibody, Zp-4 antibody, ZP3-ALPHA antibody, ZP antibody, Zp-X antibody, zona pellucida glycoprotein 4 S homeolog antibody, zona pellucida glycoprotein 4 antibody, zona pellucida glycoprotein 4 L homeolog antibody, zp4.S antibody, zp4 antibody, ZP4 antibody, zp4.L antibody, Zp4 antibody

Background: The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix.The zona pellucida is an extracellular matrix that surrounds the oocyte and early embryo. It is composed primarily of three or four glycoproteins with various functions during fertilization and preimplantation development. The nascent protein contains a N-terminal signal peptide sequence, a conserved ZP domain, a consensus furin cleavage site, and a C-terminal transmembrane domain. It is hypothesized that furin cleavage results in release of the mature protein from the plasma membrane for subsequent incorporation into the zona pellucida matrix. However, the requirement for furin cleavage in this process remains controversial based on mouse studies. Previously, this gene has been referred to as ZP1 or ZPB and thought to have similar functions as mouse Zp1. However, a human gene with higher similarity and chromosomal synteny to mouse Zp1 has been assigned the symbol ZP1 and this gene has been assigned the symbol ZP4. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 49 kDa

Gene ID: 57829

NCBI Accession: NP_067009

UniProt: Q12836