Coilin antibody

Catalog No. ABIN2463583

Product Details anti-Coilin Antibody

Target: Coilin (COIL)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Coilin antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purification: Antibody is purified by peptide affinity chromatography method.

Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human COIL.

Application Details

Application Notes: COIL antibody can be used for detection of COIL by ELISA at 1:312500. COIL antibody can be used for detection of COIL by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Add 50 ?L of distilled water. Final antibody concentration is 1 mg/mL.

Concentration: 1 mg/mL

Buffer: Antibody is lyophilized in PBS buffer with 2 % sucrose.

Handling Advice: As with any antibody avoid repeat freeze-thaw cycles.

Storage: 4 °C/-20 °C

Storage Comment: For short periods of storage (days) store at 4 °C. For longer periods of storage, store COIL antibody at -20 °C.

Target Details for Coilin

Target: Coilin (COIL)

Alternative Name: COIL (COIL Products)

Synonyms: p80c antibody, coil antibody, CG8710 antibody, Coilin antibody, Dmel\\CG8710 antibody, LOC100218802 antibody, F3F19.5 antibody, F3F19_5 antibody, CLN80 antibody, p80-coilin antibody, C79982 antibody, Cln80 antibody, p80 antibody, wu:fb08h11 antibody, sph1 antibody, Coilin antibody, coilin antibody, sphere organelles protein-like protein antibody, coilin p80 antibody, coilin L homeolog antibody, p80c antibody, COIL antibody, coil antibody, AT1G13030 antibody, Coil antibody, coil.L antibody

Background: COIL is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies.The protein encoded by this gene is an integral component of Cajal bodies (also called coiled bodies). Cajal bodies are nuclear suborganelles of varying number and composition that are involved in the post-transcriptional modification of small nuclear and small nucleolar RNAs. The N-terminus of the coilin protein directs its self-oligomerization while the C-terminus influences the number of nuclear bodies assembled per cell. Differential methylation and phosphorylation of coilin likely influences its localization among nuclear bodies and the composition and assembly of Cajal bodies. This gene has pseudogenes on chromosome 4 and chromosome 14. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Molecular Weight: 63 kDa

Gene ID: 8161

NCBI Accession: NP_004636

UniProt: P38432

Pathways: Ribonucleoprotein Complex Subunit Organization