GFP antibody (full length)

Catalog No. ABIN2464056

This anti-GFP antibody is a Mouse Monoclonal antibody detecting GFP in WB and ELISA. Suitable for Aequorea victoria.

Quick Overview for GFP antibody (full length) (ABIN2464056)

Target: GFP (Green Fluorescent Protein (GFP))

Reactivity: Aequorea victoria

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 4B10B2

Product Details anti-GFP Antibody

Binding Specificity: full length

Immunogen: Ni-NTA purified full-length recombinant GFP-His expressed in E. Coli strain BL21 (DE3).

Isotype: IgG2a

Application Details

Application Notes: Western Blot:1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Purified antibody in PBS containing 0.03 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Storage: -20 °C

Storage Comment: GFP monoclonal antibody can be stored at -20 °C, stable for one year.

Target Details for GFP

Target: GFP (Green Fluorescent Protein (GFP))

Alternative Name: GFP

Background: GFP (Green fluorescence protein) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light when excited by blue light. GFP cDNA produces a fluorescent product when expressed in prokaryotic cells, without the need for exogenous substrates or cofactors. GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technnology has considerably contributed to a greater understanding of cellular physiology.

Gene ID: 7011691

NCBI Accession: YP_002302326

UniProt: B6UPG7