To avoid confusion please note that this antibody is designed to recognise the whole protein as coded by NP_036424.2 and won't recognise the peptide found eluted from HLA-A*0201 Molecules by den Haan et al (Science. 1998 Feb 13,279(5353):1054-7, PMID: 9461441)
Purification
Antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
HMHA1 antibody was raised against a 15 amino acid synthetic peptide near the N-Terminus of HMHA1 (aa 57-71).
HMHA1
Reactivity: Human, Rat, Mouse, Cow, Dog, Pig, Hamster, Monkey, Bat, Chicken
WB
Host: Rabbit
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 128000. Western Blot: Preliminary experiments gave an approx 200 kDa band in lysates of cell lines Jurkat and Molt4 after 0.05 μg/mL antibody staining.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.