Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Purification
SH3GL2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
SH3GL2 antibody was raised against a 13 amino acid peptide near the internal region of SH3GL2.
The starting concentration recommended for Western Blot is 0.3-1μg/mL. IHC: In paraffin embedded Human Cerebral Cortex shows staining of cytoplasm in some but not all neuronal cells. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.