Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognize both reported isoforms (NP_001626.1 and NP_647477.1).
Purification
Amphiphysin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Amphiphysin antibody was raised against a 12 amino acid synthetic peptide near the internal region of Amphiphysin.
ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approximately 125 kDa band observed in mouse brain lysates (calculated MW of 76.3 kDa according to NP_001626.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Wigge et al, Mol Biol Cell. 1997 Oct,8 (10) :2003-15., PMID: 9348539). Recommended concentration: 0.1-0.5 μg/mL. A minor band of unknown identity was also consistently observed at 45 kDa . This band was successfully blocked by incubation with the immunizing peptide. Immunohistochemistry: In paraffin embedded human cerebellum shows nuclear and fibrous staning. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.