NUP85 antibody (C-Term)

Catalog No. ABIN2465261

Product Details anti-NUP85 Antibody

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Binding Specificity: C-Term

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NUP85 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Specificity: Please note that, in contrast to other anti-Pericentrin antibodies available, this ab is not expected to recognize Kendrin (also known as Pericentrin 2). This may explain why it recognizes a 75 kDa band (the expected size according to NP_079120) and not a

Purification: NUP85 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: NUP85 antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of NUP85.

Application Details

Application Notes: ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 75 kDa band observed in HeLa lysates (calculated MW of 75 kDa according to NP_079120). Recommended concentration: 1-3 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for NUP85

Target: NUP85 (Nucleoporin 85kDa (NUP85))

Alternative Name: NUP85 (NUP85 Products)

Synonyms: Pcnt1 antibody, frount antibody, pcnt1 antibody, zgc:100943 antibody, Nup75 antibody, nucleoporin 85kDa L homeolog antibody, nucleoporin 85kDa antibody, nucleoporin 85 antibody, nup85.L antibody, nup85 antibody, NUP85 antibody, Nup85 antibody

Gene ID: 79902

NCBI Accession: NP_079120

UniProt: Q9BW27