BIF-1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
BIF-1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of BIF-1.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 38 kDa band observed in lysates of human T cell line Jurkat and in human lung and placenta (calculated MW of 40.8 kDa according to NP_057093.1). Recommended concentration: 0.3-1.0 μg/mL. A minor band of unknown identity was also consistently observed at 50 kDa in Jurkat and placenta. This band was successfully blocked by incubation with the immunizing peptide.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ZBTB24
(Zinc Finger and BTB Domain Containing 24 (ZBTB24))