UBE2I antibody (N-Term)

Catalog No. ABIN2465320

Product Details anti-UBE2I Antibody

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Goat

Clonality: Polyclonal

Conjugate: This UBE2I antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Specificity: This antibody is expected to recognize all reported isoforms (NP_003336.1, NP_919235.1, NP_919236.1 and NP_919237.1).

Purification: UBE2I antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: UBE2I antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of UBE2I.

Application Details

Application Notes: ELISA: Antibody detection limit dilution 1:64,000. Western Blot: Approximately 17 kDa band observed in human kidney, human lung and human testis lysates (calculated MW of 18 kDa according to NP_003336). Recommended concentration: 0.1-0.3 μg/mL. Immunohistochemistry: In paraffin embedded human kidney, shows nuclear staining of some epithelial cells of glomeruli and renal tubules. Recommended concentration, 1 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for UBE2I

Target: UBE2I (Ubiquitin-Conjugating Enzyme E2I (UBE2I))

Alternative Name: UBE2I (UBE2I Products)

Synonyms: C358B7.1 antibody, P18 antibody, UBC9 antibody, ubc9 antibody, ube2ia antibody, zUbc9 antibody, 5830467E05Rik antibody, F830028O17Rik antibody, Ubce2i antibody, Ubce9 antibody, UbcE2A antibody, ubce9 antibody, T13J8.70 antibody, T13J8_70 antibody, UBIQUITIN-PROTEIN LIGASE antibody, ubiquitin conjugating enzyme 9 antibody, ubiquitin conjugating enzyme E2 I antibody, ubiquitin-conjugating enzyme E2Ib antibody, ubiquitin-conjugating enzyme E2L antibody, ubiquitin-conjugating enzyme E2I antibody, ubiquitin conjugating enzyme E2I S homeolog antibody, ubiquitin conjugating enzyme 9 antibody, SUMO-conjugating enzyme UBC9 antibody, UBE2I antibody, ube2ib antibody, UBE2L antibody, Ube2i antibody, ube2i.S antibody, UBC9 antibody, ubc-9 antibody, LOC108703134 antibody

Gene ID: 7329

NCBI Accession: NP_003336, NP_919235, NP_919236, NP_919237

UniProt: P63279

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Ubiquitin Proteasome Pathway