Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC)
Specificity
This antibody is expected to recognize isoform 1 (NP_005836.2) only.
Purification
ABCC4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ABCC4 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of ABCC4.
ABCC4
Reactivity: Human
WB
Host: Rabbit
Polyclonal
RB17763
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:64000. Western Blot: Approximately 170 kDa band observed in rat renal cortex membranes (calculated MW of 149 kDa according to rat NP_596902.1 and 150 kDa according to human NP_005836.2). Recommended concentration: 1-3 μg/mL. Immunohistochemistry: In paraffin embedded human prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ABCC4
(ATP-Binding Cassette, Sub-Family C (CFTR/MRP), Member 4 (ABCC4))