Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial (C-Term) antibody
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
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Binding Specificity
- C-Term
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Reactivity
- Human
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Host
- Goat
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Clonality
- Polyclonal
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Conjugate
- Un-conjugated
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Application
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
- Specificity
- This antibody is expected to recognise both reported isoforms.
- Purification
- ACADM antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Immunogen
- ACADM antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ACADM.
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- Application Notes
- ELISA: Antibody detection limit dilution 1:32000. Western Blot: Approximately 45 kDa band observed in human kidney and heart lysates (calculated MW of 46.6 kDa according to NP_000007.1). Recommended for use at 0.01-0.1 μg/mL. Immunohistochemistry: In paraffin embedded human liver shows mitochondrial staining in hepatocytes. Recommended concentration, 3-5 μg/mL.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 500 μg/mL
- Buffer
- Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Minimize freezing and thawing.
- Storage
- -20 °C
- Storage Comment
- Aliquot and store at -20 °C.
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- Target
- Medium-Chain Specific Acyl-CoA Dehydrogenase, Mitochondrial
- Alternative Name
- ACADM
- Synonyms
- ACAD1 antibody, MCAD antibody, MCADH antibody, AU018656 antibody, acyl-CoA dehydrogenase medium chain antibody, acyl-Coenzyme A dehydrogenase, medium chain antibody, acyl-CoA dehydrogenase, C-4 to C-12 straight chain antibody, ACADM antibody, Acadm antibody
- Gene ID
- 34
- NCBI Accession
- NP_000007
- UniProt
- P11310
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