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AIRE antibody (C-Term)
AIRE
Reactivity: Human
WB, ELISA, IHC, ChIP
Host: Goat
Polyclonal
unconjugated
Product Details anti-AIRE Antibody
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Target
See all AIRE Antibodies
AIRE
(Autoimmune Regulator (AIRE))
Binding Specificity
All epitopes for AIRE antibodies
C-Term
Reactivity
All reactivities for AIRE antibodies
Human
Host
All hosts for AIRE antibodies
Goat
Clonality
All clonalities for AIRE antibodies
Polyclonal
Conjugate
All conjugates for AIRE antibodies
This AIRE antibody is un-conjugated
Application
All applications for AIRE antibodies
Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP)
Specificity
This antibody is expected to recognize isoforms 1 and 2 (NP_000374.1 and NP_000649.1 respecively).
Purification
AIRE antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
AIRE antibody was raised against a 12 amino acid synthetic peptide near the C-Terminus of AIRE.
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Application Details
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Application Notes
ELISA: Antibody detection limit dilution 1:32000. Western Blot: Appr. 60 kDa in transfected HEK293 lysates (calculated MW of 57.7 kDa according to NP_000374.1). Recommended concentration: 0.01-0.03 μg/mL. Immunohistochemistry: In paraffin embedded human Thymic medula shows staining of select nuclei. Recommended concentration, 2.5 μg/mL.
Restrictions
For Research Use only
Handling
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Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target Details for AIRE
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Target
AIRE
(Autoimmune Regulator (AIRE))
Alternative Name
AIRE (AIRE Products )
Synonyms
AIRE1 antibody, APECED antibody, APS1 antibody, APSI antibody, PGA1 antibody, autoimmune regulator antibody, autoimmune regulator (autoimmune polyendocrinopathy candidiasis ectodermal dystrophy) antibody, AaeL_AAEL013794 antibody, CpipJ_CPIJ001208 antibody, CpipJ_CPIJ001209 antibody, AIRE antibody, Aire antibody
Gene ID
326
NCBI Accession
NP_000374 , NP_000649
UniProt
O43918
Pathways
Chromatin Binding
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