Western Blotting (WB), Immunohistochemistry (IHC), ELISA
Specificity
This antibody may cross-react with ALDH1A2 (GeneID 8854) with one residue difference from this design and with ALDH2 (GeneID 217) with two residues difference from this design.
Purification
ALDH1A1 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
ALDH1A1 antibody was raised against a 14 amino acid synthetic peptide near the C-Terminus of ALDH1A1.
ALDH1A1
Reactivity: Human
WB, ELISA, IHC (p), IP
Host: Mouse
Monoclonal
1G6
unconjugated
Application Notes
ELISA: Antibody detection limit dilution 1:16,000. Western Blot: Approximately 55 kDa band observed in human liver lysates (calculated MW of 54.9 kDa according to NP_000680.2). Recommended concentration: 0.03-0.1 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
ALDH1A1
(Aldehyde Dehydrogenase 1 Family, Member A1 (ALDH1A1))