NLRP3 antibody (C-Term)

Catalog No. ABIN2465561

The Goat Polyclonal anti-NLRP3 antibody is suitable to detect NLRP3 in samples from Human. It has been validated for ELISA and FACS.

Quick Overview for NLRP3 antibody (C-Term) (ABIN2465561)

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This NLRP3 antibody is un-conjugated

Application: ELISA, Flow Cytometry (FACS)

Product Details anti-NLRP3 Antibody

Binding Specificity: C-Term

Purification: Cryopyrin antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogen: Cryopyrin antibody was raised against a 13 amino acid synthetic peptide near the C-Terminus of Cryopyrin.

Application Details

Application Notes: ELISA: Antibody detection limit dilution >1:32000. Western Blot: No signal obtained yet but low background observed in Daudi, A431, Jurkat, U937 and MOLT-4 lysates at up to 1 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 500 μg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Minimize freezing and thawing.

Storage: -20 °C

Storage Comment: Aliquot and store at -20 °C.

Target Details for NLRP3

Target: NLRP3 (NLR Family, Pyrin Domain Containing 3 (NLRP3))

Alternative Name: Cryopyrin

Gene ID: 114548

NCBI Accession: NP_004886

UniProt: Q96P20

Pathways: Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Inflammasome