E2-EPF antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
E2-EPF antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of E2-EPF.
UBE2S
Reactivity: Human
WB, IF
Host: Mouse
Polyclonal
unconjugated
Application Notes
ELISA: Antibody detection limit dilution >1:32,000. Western Blot: Approximately 35-40 kDa band observed in human liver lysate at 1 ? of primary, this band was successfully blocked by incubation with the immunizing peptide. Please note that the band we observe is different from the predicted size of approximately 25 kDa according to NP_055316.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.