ELISA, Western Blotting (WB), Immunohistochemistry (IHC)
Purification
FBL2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
FBL2 antibody was raised against a 13 amino acid synthetic peptide near the N-Terminus of FBL2.
ELISA: Antibody detection limit dilution 1:32,000. Western Blot: Approximately 48 kDa band observed in human brain lysates (calculated MW of 47.1 kDa according to NP_036289.2). Recommended concentration: 0.3-1.0 μg/mL. IHC: In paraffin embedded Human Cerebral Cortex shows cytoplasm staining in neuronal bodies. Recommended concentration, 5-10μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Minimize freezing and thawing.
Storage
-20 °C
Storage Comment
Aliquot and store at -20 °C.
Target
FBXL20
(F-Box and Leucine-Rich Repeat Protein 20 (FBXL20))