Western Blotting (WB), ELISA, Immunohistochemistry (IHC)
Specificity
This antibody is expected to recognise both reported isoforms.
Purification
FOXA2 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
FOXA2 antibody was raised against an 11 amino acid synthetic peptide near the C-Terminus of FOXA2.
ELISA: Antibody detection limit dilution 1:4000. Western Blot: Approximately 60 kDa band observed in human liver lysates (calculated MW of 48.3 kDa according to NP_068556.1and NP_710141.1). The observed molecular weight corresponds to earlier findings in literature (Wang et al, J Biol Chem. 2002 May 17,277 (20) :17564-70., PMID: 11875061). Recommended concentration: 1-3 μg/mL.
Restrictions
For Research Use only
Format
Liquid
Concentration
500 μg/mL
Buffer
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.